The percentage of motile spermatozoa in a thermoresistance test on d2 showed a significant drop using ≤70% EC (P=0.047) and ≥140% EC (P=0.004). Total sperm motility with 100% EC differed significantly from ≤70% EC (P<0.001) and 200% EC (P<0.001) on day 1 (d1) and d4, respectively. The refractive index for the correctly prepared extender was 4.6☐.0☋x, corresponded to 316☑6mOsmkg(-1), and correlated highly with osmolality (r=0.99 P<0.001). Twelve boar ejaculates were evaluated for semen quality. For this the refractive index and osmolality of BTS extender concentrations (EC) were recorded in 10%-steps from 50% to 150% and 200% of the correct amount. The study was published online, ahead of printing, in the Journal of Animal Science, August 2013.A study was performed to see if refractometry can be used as a new quality control tool for boar semen extenders. New semen extenders and other modified preservation strategies require sensitive testing for essential sperm functions. Extenders are continuously developed to protect boar spermatozoa from chilling injury. The research is relevant as spermatozoa, especially those of the porcine species, are highly susceptible to in vitro-chilling and -ageing. In short, the researchers conclude that “ assessment of specific reactivity to capacitating stimuli appears to be a sensitive tool for detection of extender-dependent alterations in functionality of chilled boar spermatozoa.”
#MINITUBE BTS PLUS#
Fertility parameters did not differ in semen stored up to 48 hours at 10☌ in Androstar Plus compared to controls stored at 17☌ in BTS.”
#MINITUBE BTS TRIAL#
“A field insemination trial with 778 sows was performed to relate in vitro results to fertility. Assessment of extent of phopholipid disorder under capacitating and control conditions by use of the merocyanine staining did not reveal any significant extender-related differences. “There was a higher loss of response in semen stored hypothermically in the standard extender BTS compared to Androstar Plus.
Based on the loss of low-calcium live cells in a kinetic approach, the specific response to capacitation stimuli was determined. “Washed sub-samples were incubated in capacitating medium (Tyrode) and control medium and assessed for intracellular calcium concentration and integrity of plasma membranes using a flow cytometer. Motility and membrane integrity remained at high levels, except for lower values when stored in BTS at 5☌. Semen was analysed at 24 and 96 hours of storage. The researchers report: “They were kept for three hours at 22☌ or stored at 17☌, 10☌ and 5☌.
In the trial, semen was diluted in two different extenders: When using artificial insemination, in vitro, capacitation can occur by incubation. This is a phase of maturation of spermatozoa – usually happening after ejaculation. The researchers, attached to the University of Veterinary Medicine in Hanover, Germany and the University of Cambridge, UK, zoomed in on the concept of ‘capacitation’ for their research. Joint German and English research has paved the way for a new method of determining the efficacy of certain extenders when storing and chilling boar semen.
Research: New way of examining boar extender efficacy
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